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生物醫學暨檢驗雜誌第24卷第1期 JOURNAL OF BIOMEDICAL AND LABORATORY SCIENCES Vol. 24, No. 1, 2012

原著論文 Original Articles

以牛樟芝餵食小鼠28天亞急性毒性試驗之組織病理與生化分析
Histopathology and Biochemistry Analysis in a 28-day Subacute Toxicological Assessment of Antrodia Cinnamomea in BALB/c Mice
吳銘芳 李美慧 黃韶顏 劉嘉又 鍾明燈 常傳訓 敖曼冠 廖年捷 薛樹清 呂旭峰
Ming-Fang Wu, Mei-Hui Lee, Shau-Yen Huang, Jia-You Liu, Ming-Teng Chung, Chuan-Hsun Chang, Man-Kuan Au, Nien-Chieh Liao, Shu-Ching Hsueh, Hsu-Feng Lu
生檢雜誌2012;24:5-11 【Abstract】 【摘要】看PDF需登入會員
中文摘要
在台灣,非處方之中草藥常被用來治療腹痛、高血壓與肝腫瘤,並逐漸被全世界所採用。在東方世界,這些中草藥之中又以牛樟芝為最有名的調理聖品,因此提供毒理檢查已確定食品安全性有其必要。本研究建立小鼠(BALB/c mice)28天口服亞急性毒性分析模式,在每組十隻老鼠且雌雄分別下,以16.7、833.3與1666.7毫克/公斤/天三種劑量連續28天口服餵食,觀察其亞急性毒性反應。各組老鼠在實驗最後仍皆存活,且實驗組老鼠之血液與生化檢驗結果皆與對照組無統計學差異,進ㄧ部的肝臟、腎臟及脾臟的組織切片分析顯示各組皆未有明顯的病理與毒理變化。因此,從此實驗結果可瞭解到未觀察到毒性的劑量至少為1666.7 mg/kg/天。
關鍵詞:
Abstract
Non-prescriptional use of medicinal herbs is highly prevalent to treat abdominal pain, hypertension and hepatocellular carcinoma in Taiwan and increasing worldwide. Among them, Antrodia cinnamomea was the most famous modalities being consumed especially among Oriental. Since the use of Antrodia cinnamomea in herbal medicines is increased, toxicity studies are warranted to confirm the safe use of Antrodia cinnamomea. Based on the 28-day subchronic oral toxicity studies of Antrodia cinnamomea extract in male and female BALB/c mice, the extract at doses of 16.7, 833.3, and 1666.7mg/kg/day were administered by oral gavage to groups of mice (10 mice/dose) for 28 consecutive days and sterile water was used as control. All animals survived to the end of the study. No significant differences were found in hematology and serum biochemistry parameters among the control and treatment groups. Further histological examination in liver, kidney and spleen showed no pathological changes among all groups. These results suggest that the no-observed-adverse-effect level (NOAEL) of the extract for male and female mice is considered to be 1666.7 mg/kg/day.
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